Microbiology Lab-2021 Exercise 1.2: Understanding Sugar and Salt Concentrations

Questions:

1. What weight of sugar and salt are required to prepare 30 ml 30% solutions of each solute?

2. What are the percent concentrations of glucose and sodium chloride in TSB?

3. How many grams of glucose and sodium chloride are present in 5 mL of 2X TSB?

4. Complete the following table for sugar and salt concentrations.

Answers:

1. To prepare 30 ml 30% solutions of sugar and salt, you would need 9 grams of sugar and 9 grams of salt.

2. Glucose concentration in TSB is approximately 22.73%, while sodium chloride concentration is around 45.45%.

3. There are 5 grams of glucose and 10 grams of sodium chloride present in 5 mL of 2X TSB solution.

Explanation:

When preparing a 30% solution of sugar and salt in 30 ml, the calculation is straightforward. For both sugar and salt, you will require 9 grams of each to achieve the desired concentration.

In TSB, the percent concentrations of glucose and sodium chloride are calculated based on the provided formula per liter. The glucose concentration is approximately 22.73%, while sodium chloride concentration is around 45.45% in TSB. When dealing with 2X TSB, which has double the concentration, the values are doubled accordingly.

To determine the amount of glucose and sodium chloride present in 5 mL of 2X TSB, you can use the formula provided in the lab manual. There are 5 grams of glucose and 10 grams of sodium chloride in 5 mL of 2X TSB. Understanding these concentrations and calculations is vital for accurate experimentation and analysis in microbiology labs.