Growing Bacterium Experiment: Calculating Final Volume and Concentration
Experiment Setup:
You are growing a bacterium that requires the amino acid, arginine for growth. In order to create your growth medium, you add 5ml of sterile 50mM arginine to 45ml of LB broth.
a. Final Volume of Growth Medium:
To calculate the final volume of your growth medium, you need to add the volumes of arginine and LB broth together.
Volume of arginine: 5ml
Volume of LB broth: 45ml
Final answer: The final volume of the growth medium is 50ml.
b. Final Concentration of Arginine:
To determine the final concentration of arginine in the growth medium, you need to consider the initial concentration and dilution factor.
Initial concentration of arginine: 50mM
Dilution factor: 1 (no additional dilution is mentioned)
Final answer: The final concentration of arginine in the growth medium is 50mM.
Explanation: To calculate the final volume of the growth medium, we add the volumes of arginine and LB broth together: Total volume of growth medium = 5ml + 45ml = 50ml. To determine the final concentration of arginine, we use the formula: Final concentration = Initial concentration / Dilution factor. Substituting the values, we get a final concentration of 50mM.
11.You are growing a bacterium that requires the amino acid, arginine for growth. You create your growth medium by adding 5ml of sterile 50mM arginine to 45ml of LB broth. a. What is the final volume of your growth medium? b. What is the final concentration of arginine in the growth medium? Final answer: The final volume of the growth medium is 50ml. The final concentration of arginine in the growth medium is 50mM. Explanation: To calculate the final volume of the growth medium, we need to add the volumes of the arginine and LB broth: Volume of arginine: 5ml Volume of LB broth: 45ml Adding these volumes together, we get: Total volume of growth medium = 5ml + 45ml = 50ml To calculate the final concentration of arginine, we need to consider the initial concentration and dilution factor: Initial concentration of arginine: 50mM Dilution factor: 1 (since no additional dilution is mentioned) Using the formula: Final concentration = Initial concentration / Dilution factor Substituting the values: Final concentration of arginine = 50mM / 1 = 50mM.