Experiment Results: Cali and Spartan Bacteria Break Down H2O2
What are the experiment results of adding Cali and Spartan bacteria to a buffer containing H2O2?
How do Cali and Spartan bacteria contribute to the breakdown of H2O2 in the buffer?
Experiment Results:
Buffer alone: High levels of H2O2
Buffer with Cali: Decreased levels of H2O2
Buffer with Spartan: Decreased levels of H2O2
Interpretation: Both Cali and Spartan bacteria contribute to the breakdown of H2O2, resulting in decreased levels. Cali, with its high catalase levels, and Spartan, with its high peroxidase levels, effectively reduce the concentration of H2O2 in the buffer. The absence of ferrous iron in the buffer indicates that the observed decrease in H2O2 is due to enzymatic activity rather than iron-mediated reactions.
In this experiment, the addition of Cali and Spartan bacteria to a buffer containing H2O2 resulted in decreased levels of H2O2 compared to the buffer alone. The high catalase levels in Cali and high peroxidase levels in Spartan contribute to the breakdown of H2O2 in the buffer. This enzymatic activity by both bacteria effectively reduces the concentration of H2O2, highlighting their role in the process.
A buffer is a solution that helps maintain a stable pH level by resisting changes in acidity or alkalinity. It consists of a weak acid and its conjugate base (or a weak base and its conjugate acid) that work together to neutralize any added acid or base. The buffer used in this experiment containing H2O2 serves as the medium in which Cali and Spartan bacteria are introduced, allowing the measurement of H2O2 levels and evaluating the impact of the bacteria on its breakdown.